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. 2016 Jan 12;7(6):7268–7279. doi: 10.18632/oncotarget.6896

Figure 2. ART-838 inhibited human acute leukemia cell cycle progression more potently than did AS.

Figure 2

MOLM14 cells treated with ART-838 (0.1 μM) or AS (5 mM) for 24h were analyzed for cell cycle distribution by measuring DNA synthesis (BrdU incorporation) and DNA content (7-AAD) via flow cytometry, using the FL3 and FL4 channels of an Accuri C6 flow cytometer (BD Biosciences). After cell debris was gated out using FSC/SSC parameters, 10,000 cellular events were collected per sample. Data was analyzed and fluorescence compensation performed using FlowJo software, version 10 (Tree Star, Ashland, OR). A. Representative plots from a single experiment. B. Quantification of the percentage cells in each phase of the cell cycle from three independent flow cytometry experiments (**, p<.01 vs control; ***, p<.001 vs control).