. Author manuscript; available in PMC: 2016 May 19.

Published in final edited form as: SOJ Microbiol Infect Dis. 2015 Dec 3;3(3):10.15226/sojmid/3/3/00141. doi: 10.15226/sojmid/3/3/00141

Table 1.

Bacterial strains and plasmids used in this study

Strains	Genotype	Source/reference
*Vibrio cholerae*
0395	01 classical, Str^r	(19)
0395 ΔacfB	ΔacfB, Str^r	This study
0395 ΔacfB/pACFB	ΔacfB/pacfB, Str^r, Amp^r	This study
0395 ΔtoxR	ΔtoxR, Str^r	(19)
0395 ΔtcpI	ΔtcpI, Str^r	This study
0395 ΔcheW1	ΔcheW1, Str^r	This study
0395 ΔcheW2	ΔcheW2, Str^r	This study
0395 ΔcheW3	ΔcheW3, Str^r	This Study
*Escherichia coli*
Top10	F⁻mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1deoR araD139 Δ(ara-leu)7697 galUgalKrpsL (Str^r) endA1 nupG	Invitrogen
DH5α (λpir)	supE4 DlacU169 (Φ80 lacZDM15) hsdR17 recA1 endA1 gyrA96thi-1 relA1 λpir	Invitrogen
Sm10 (λpir)	thi-1 thrleutonAlacYsupErecA::RP4-2Tc::Mu λpir R6K	(37)
Sy327(λpir)	Δ(lac-pro) arE(Am) rifnalA recA56 λpir	(37)
Plasmids
pBAD	colE1 ori; araBAD promoter; Amp^r	Invitrogen
pACFB	acfB in pBAD; Amp^r	This study
pCVD442	R6K ori;, mobRP4, bla, sacB	(38)
pGP704	R6K ori; Amp^r	(37)