Table 1. Retention times, mass spectral data and wavelength of maximal absorption (λmax) for the chemical constituents found in hydroethanolic extracts from Turnera diffusa .
| Peak | Rt min | λmax (nm) | (ESI+, m/z) | (ESI-, m/z) | Proposed structure | |
|---|---|---|---|---|---|---|
|
|
|
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| [M + H]+ | [M – H]- | MS/MS | ||||
| 1 | 23.5 | 265 and 345 | - | 593 | 473 (100), 429 (40), 357 (50) and 327 (80) | Luteolin-6-C-glucosyl-2”-O-rhamnoside |
| 2 | 24.0 | 265 and 345 | - | 567 | 521 | Luteolin-8-C-(6”-O-p-benzoyl)-glucoside |
| 3 | 25.5 | 260 and 355 | - | 641 | 317 | Myricetin-3-O-diglucoside |
| 4 | 26.2 | ND | - | 743 | 605 (100) | Luteolin-8-C-(O-p-feruloyl-2”-O-benzoyl) glucoside |
| 5 | 27.7 | 260 and 355 | - | 625 | 301 | Quercetin-3-O-diglucoside |
| 6 | 28.2 | 260 and 355 | - | 655 | 331 | Laricitin-3-O-diglucoside |
| 7 | 30.1 | ND | - | 639 | 575 | Quercetin-O-caffeoyl glucuronide |
| 8 | 30.8 | 252, 265 and 357 | - | 669 | 345 | Syringetin-3-O-diglucoside |
| 9 | 33.6 | 270 and 345 | 577 | 575 | 411 (100), 301 (60) and 285 (20) | Luteolin-8-C-[6-deoxy-2-O-rhamnosyl]-xylo-hexos-3-uloside, |
| 10 | 35.5 | 270 and 340 | 561 | 559 | 395 (100), 321 (20) and 269 (20) | Apigenin-8-C-[6-deoxy-2-O-rhamnosyl]-xylo-Hexos-3-uloside, |
| 11 | 37.0 | 270 and 315 | 579 | 577 | 269 | Apigenin-7-O-(6”O-p- coumaroyl)-glucoside |
Rt min: minimum retention times; ESI: electrospray ionization; M-H: deprotonated molecule; M+H: protonated molecule; MS: mass spectrometry; ND: not determined.