(A) Transcript levels of 7-day-old seedlings as indicated. Error bars represent SE. (B) After induction of OBE3 overexpression, mRNA levels of WUS are increased, whereas mRNA levels of CLV3, STM, and ARR7 are unchanged in 7-day-old seedling. Error bars represent SD. (C) pWUS:GUS expression in 6-day-old obe3-1 obe4-1/+ seedlings is confined to the OC as in the wild type. (D) WUS mRNA is undetectable by in situ hybridization in obe3-1 obe4-1/+ floral meristems of 30-day-old plants. (E) WUS overexpression upregulates OBE3 and OBE4 mRNA levels in 7-day-old seedlings. ARR7 expression is used as a control. Error bars represent SD. Relative mRNA levels compared to the mock control are shown.*,p<0.05, calculated from Cp’ values; ***, p<0.001, calculated from Cp’ values.