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. 2016 May 19;11(5):e0155657. doi: 10.1371/journal.pone.0155657

Table 1. obe3-2 enhances the meristem defects of weak and intermediate wus alleles.

% of phenotype (of germinated seeds)
seedling (10 DAG) shoot (51DAG)
genotype of mother plant n ng wt-like wus-7 like wus-1 like retard1 wt-like wus-6 like wus-1 like disorganized leaves, no stem arrest2
Ler 244 42 99.5 0.0 0.0 0.5 99.0 0.0 0.0 0.0 1.0
obe3-2 51 2 98.0 0.0 0.0 2.0 98.0 0.0 0.0 0.0 2.0
wus-1/+ 202 3 69.9 0.0 28.1 2.0 68.8 1.5 24.6 4.0 1.1
obe3-2 wus-1/+ 205 2 75.9 0.0 20.7 3.4 69.5 3.9 1.5 19.7 5.4
wus-6/+ 245 0 73.5 0.0 25.3 1.2 73.1 25.7 0.0 0.0 1.2
obe3-2 wus-6/+ 212 1 74.4 0.0 24.2 1.4 74.4 0.0 0.0 23.7 1.9
wus-7/+ 237 2 94.0 5.6 0.0 0.4 79.6 19.5 0.0 0.0 0.9
obe3-2 wus-7/+ 228 4 93.8 4.9 0.4 0.9 75.4 19,2 0.0 4.5 0.9

Seedling phenotype classes: wt-like, shoot meristem forming a rosette of leaves; wus-7-like; shoot meristem termination after true leaves have been formed, wus-1-like: shoot meristem termination without any leaves.

Shoot phenotype classes: wt-like, rosette, indeterminate inflorescence; wus-6-like, disorganized leaves, indeterminate inflorescence; wus-1-like, disorganized leaves, stop-and-go inflorescence rarely forming flowers.

DAG, days after germination; n, number of plants analyzed; ng, not germinated;

1, retard: small whitish seedlings with retarded growth and cotyledons only.

2, arrest: plants stopped development at seedling stage.

Chi-square test results for the seedling phenotype difference between:

wus-1/+ vs obe3-2 wus-1/+, p>0.05, not significant

wus-6/+ vs obe3-2 wus-6/+, p>0.05, not significant

wus-7/+ vs obe3-2 wus-7/+, p>0.05, not significant

Chi-square test results for the shoot phenotype difference between:

wus-1/+ vs obe3-2 wus-1/+, p<0.0001, highly significant

wus-6/+ vs obe3-2 wus-6/+, p<0.0001, highly significant

wus-7/+ vs obe3-2 wus-7/+, 0.01<p<0.05, significant