Table 1. obe3-2 enhances the meristem defects of weak and intermediate wus alleles.
% of phenotype (of germinated seeds) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seedling (10 DAG) | shoot (51DAG) | ||||||||||
genotype of mother plant | n | ng | wt-like | wus-7 like | wus-1 like | retard1 | wt-like | wus-6 like | wus-1 like | disorganized leaves, no stem | arrest2 |
Ler | 244 | 42 | 99.5 | 0.0 | 0.0 | 0.5 | 99.0 | 0.0 | 0.0 | 0.0 | 1.0 |
obe3-2 | 51 | 2 | 98.0 | 0.0 | 0.0 | 2.0 | 98.0 | 0.0 | 0.0 | 0.0 | 2.0 |
wus-1/+ | 202 | 3 | 69.9 | 0.0 | 28.1 | 2.0 | 68.8 | 1.5 | 24.6 | 4.0 | 1.1 |
obe3-2 wus-1/+ | 205 | 2 | 75.9 | 0.0 | 20.7 | 3.4 | 69.5 | 3.9 | 1.5 | 19.7 | 5.4 |
wus-6/+ | 245 | 0 | 73.5 | 0.0 | 25.3 | 1.2 | 73.1 | 25.7 | 0.0 | 0.0 | 1.2 |
obe3-2 wus-6/+ | 212 | 1 | 74.4 | 0.0 | 24.2 | 1.4 | 74.4 | 0.0 | 0.0 | 23.7 | 1.9 |
wus-7/+ | 237 | 2 | 94.0 | 5.6 | 0.0 | 0.4 | 79.6 | 19.5 | 0.0 | 0.0 | 0.9 |
obe3-2 wus-7/+ | 228 | 4 | 93.8 | 4.9 | 0.4 | 0.9 | 75.4 | 19,2 | 0.0 | 4.5 | 0.9 |
Seedling phenotype classes: wt-like, shoot meristem forming a rosette of leaves; wus-7-like; shoot meristem termination after true leaves have been formed, wus-1-like: shoot meristem termination without any leaves.
Shoot phenotype classes: wt-like, rosette, indeterminate inflorescence; wus-6-like, disorganized leaves, indeterminate inflorescence; wus-1-like, disorganized leaves, stop-and-go inflorescence rarely forming flowers.
DAG, days after germination; n, number of plants analyzed; ng, not germinated;
1, retard: small whitish seedlings with retarded growth and cotyledons only.
2, arrest: plants stopped development at seedling stage.
Chi-square test results for the seedling phenotype difference between:
wus-1/+ vs obe3-2 wus-1/+, p>0.05, not significant
wus-6/+ vs obe3-2 wus-6/+, p>0.05, not significant
wus-7/+ vs obe3-2 wus-7/+, p>0.05, not significant
Chi-square test results for the shoot phenotype difference between:
wus-1/+ vs obe3-2 wus-1/+, p<0.0001, highly significant
wus-6/+ vs obe3-2 wus-6/+, p<0.0001, highly significant
wus-7/+ vs obe3-2 wus-7/+, 0.01<p<0.05, significant