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. 2016 May 19;11(5):e0155687. doi: 10.1371/journal.pone.0155687

Fig 1. Generation and detection of tdTomato BAC reporter rats.

Fig 1

(A) The transgene, which was inserted into the mouse ROSA26 BAC clone, comprised the CAG promoter, a cassette for the neomycin resistance gene with four STOP signal repeats (pA) flanked by loxP sites, and a sequence containing the tdTomato open reading frame. After Cre-mediated recombination, tdTomato expression was under the regulation of the generic CAG promoter. (B) PCR analysis of genomic DNA. The 726-bp band, which is recognized by the designed primer sets targeting the PGK-neo cassette located in between two loxP sequences, was present in the tdTomato reporter rats but not in the wild-type rats.