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. 2016 May 19;11(5):e0155687. doi: 10.1371/journal.pone.0155687

Fig 2. AAV2-Cre-mediated reporter gene expression in the brain.

Fig 2

(A) Co-localization of Cre-immunoreactive nuclei (green) and tdTomato immunoreactivity (red) in the same cells [merged image and the 3D reconstruction image (A′)]. (B) Quantitative analysis for the in vivo recombination efficiency. 525(+)/590(-) or 525(+)/590(+) cells were counted from immunostained brain slices (n = 12). **, p < 0.001, Mann-Whitney U-test. (C) Morphology of neurons in the hippocampal CA1 region. The projecting axons were traceable to fine branches (fixed 20-μm slice without enhancement). (D) Enlarged image of CA1 dendrites. Note that fine structures such as spines can be visualized clearly.