Fig 3. Minimum spanning tree of the isolates of 76 S. sonnei isolates from Guizhou Province based on the Multiple-Locus Variable number tandem repeat (VNTR) Analysis (MLVA).
The minimum spanning tree was constructed with the MLVA profiles of 76 isolates from Guizhou Province using BioNumerics software. Minimum spanning tree algorithm of BioNumerics software was used to construct a minimum spanning tree (MST) to determine phylogenetic pattern. Each circle corresponds to a MLVA type. The shadow zones in different color correspond to different MLVA clusters (MC), including MC12 and MC7. The size of the circle is proportional to the number of the isolates, and the color within the cycles represents the serotypes of the isolates. The corresponding color, number of isolates and serotypes were shown alongside the minimum spanning tree on the right.