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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Alcohol Clin Exp Res. 2015 Aug 6;39(9):1619–1631. doi: 10.1111/acer.12808

Figure 1.

Figure 1

TIRF imaging of BK channels in hippocampal neurons. Representative TIRF images showing BK perimembrane expression in clusters and diffuse localization in hippocampal neurons. (A) Left: TIRF imaging of endogenous BK channels after immunofluorescence, right: GM1 staining showing the outline of the neuron. (B) Measurements of clusters. Left: higher magnification view of the boxed area in (A). Note the bright puncta (clusters) and diffuse staining, Right: binary image showing thresholding used for measuring clusters. (C) Histogram showing the size distribution of BK channel clusters (2618 clusters were quantified from 134 neurons).