Cluster size, # of cluster per area and % surface area do not change after time-dependent incubations with EtOH. Hippocampal neurons were incubated with EtOH for 10-min (control N=46, EtOH N=50 neurons), 3-hrs (control N=53, EtOH N=50 neurons), 6-hrs (control N=35, EtOH N=35 neurons) and 24-hrs (control N=47, EtOH N=48 neurons). Neurons were fixed and stained immediately against the BK channel afterwards. (A) Size of the clusters, (B) number of clusters per area unit and (C) % surface area were quantified for each cell imaged with TIRF microscopy. Values from EtOH treated cells were compared to their own control groups and no significant differences were found between pairs. Unpaired t-test, p > 0.05. Error bar is ±SEM.