Fig 5. CD163 and IBA-1 Dual Fluorescence Labeling.

Shown are the results of dual fluorescence labeling for CD163-li (pseudocolored cyan) and IBA-1-li (pseudocolored magenta) in a lymph node cortical follicle and medulla from the control calf and representative infected calves. In both calves, most CD163-li cells were co-labeled by IBA-1-li but not all IBA-li cells were co-labeled with CD163-li. IBA-1-li cells without definitive CD163-li were most frequent in the control calf lymph parafollicular regions. Scale bar: 20 μm.