Table 1.
Description of 30 new microsatellite markers developed in Handroanthus billbergii.
| Locusa | Primer sequences (5′–3′) | Repeat motif | Allele size range (bp) | GenBank accession no. |
| mTb002 | F: GGAACGTGCTAGTGTGTGTG | (GT)15 | 235–297 | KT715664 |
| R: AGAGAGTGAGTTGCAACAAAAGT | ||||
| mTb003 | F: GACCTTCTGCTTGTGTTCC | (TG)15 | 142–157 | KT715665 |
| R: CTGTAAGTGTTAATTCTGCTGCT | ||||
| mTb005 | F: GCAAGGGTGGGGTAGAG | (TC)16 | 186–218 | KT715666 |
| R: GGGCAACGCATCTTGT | ||||
| mTb006 | F: GGGCTTCACATTGGTTG | (TC)18 | 162–172 | KT715667 |
| R: GGCATTTCCCAAGAACA | ||||
| mTb011 | F: TAATTTCCGGGTGCAGA | (AG)17 | 167–193 | KT715668 |
| R: CACTGGTCTCTCACATTTCAC | ||||
| mTb013 | F: CTTCTCATTCATTTTGGTGG | (TC)15 | 354–368 | KT715669 |
| R: GCTTCAACACTTTCACACATC | ||||
| mTb014 | F: TCAGTGCAACTCCATTCC | (CT)15 | 247–259 | KT715670 |
| R: AATGAACGGCATCATCTTT | ||||
| mTb015 | F: AGCAACACAAGGAGCATTT | (TC)21 | 275–301 | KT715671 |
| R: GTCAGACCCAATAACTTACCTTC | ||||
| mTb016 | F: CCAGCCTCAGTTTCAGTTC | (AG)15 | 126–156 | KT715672 |
| R: CCATTGGGATCTCTGCTT | ||||
| mTb017 | F: TGAACATGGAACAGAGCAA | (AG)16 | 216–274 | KT715673 |
| R: AGCCCAAGCGGATACA | ||||
| mTb018 | F: GTGGTGCAGCGACTTCT | (CT)15 | 230–238 | KT715674 |
| R: ACATCATCGTCATCCTCATC | ||||
| mTb019 | F: CAAATAAAAGTCATAGCAGAGG | (AG)19 | 272–312 | KT715675 |
| R: TGGCATTGAACACAACTC | ||||
| mTb020 | F: CATTGACTCGTTGTCCC | (AG)15 | 305–341 | KT715676 |
| R: GATCCTACAGTCTCACATAGAAG | ||||
| mTb021 | F: ATTGTTGATGAAGGGCAAA | (TC)18 | 269–277 | KT715677 |
| R: GGGCAAGGCTAAAGGAA | ||||
| mTb022 | F: AGATCCACGAACCCAAAA | (AG)15 | 202–240 | KT715678 |
| R: GAACGCCGAAGTGTGAG | ||||
| mTb023 | F: AGCGCAATGTGATAAGAGCT | (AC)14 | 252–262 | KT715679 |
| R: GCCCTTCATTCTTGGTGAGC | ||||
| mTb024 | F: CCATTTGCTTGCCTTACCCA | (TA)12 | 199–209 | KT715680 |
| R: AAGCAAACAACCACTCTGCA | ||||
| mTb025 | F: CAAAGTGAGAGGAACTGAAAA | (AT)11 | 354–368 | KT715681 |
| R: GGACACGAGCCAAGAAG | ||||
| mTb027 | F: TTTTCACAACCAGTAACTTC | (ATT)15 | 165–201 | KT715682 |
| R: GGTGTTTGGCATTACTTT | ||||
| mTb028 | F: TGGCAAGGACATAATCTTCAAGA | (TAA)17 | 185–215 | KT715683 |
| R: AAAACCCCAAATTCACTCCCT | ||||
| mTb029 | F: AACGAAAGAGGCGAGGT | (TTA)13 | 273–282 | KT715684 |
| R: TCCACCCATGTCCAATC | ||||
| mTb031 | F: TGCAAGTCCTGGGAAGCATA | (CTT)12 | 194–215 | KT715685 |
| R: GCACGAACAGAATGTCCAGG | ||||
| mTb032 | F: CGTCGAATATCTAGTGTGGG | (AAT)13 | 189–213 | KT715686 |
| R: ACAGATGAAGAGAAAACCAAAG | ||||
| mTb033 | F: ACAAGGAAGTAAATTGCAACTCG | (ATT)15 | 171–205 | KT715687 |
| R: ACCAGACTCCAAACACGACT | ||||
| mTb035 | F: TCCTAATTCACCAACTTCC | (ATT)15 | 151–183 | KT715688 |
| R: GTCTGTAAGCCACATAGACTG | ||||
| mTb036 | F: CGACTTCCACCATCCAA | (TTA)14 | 149–170 | KT715689 |
| R: CCTTTCTTTTGCAGCCC | ||||
| mTb037 | F: TCTTGTTGGGAATAATTGGA | (AAT)15 | 235–265 | KT715690 |
| R: GCATTAGGCAAAATTCGAG | ||||
| mTb041 | F: CGACATTCTTGCTCCCAATCA | (TAA)16 | 221–260 | KT715691 |
| R: AAACAGCGGCAAGAAAGGTT | ||||
| mTb043 | F: GGTCTAGCACGTGACTAACC | (ATT)16 | 232–275 | KT715692 |
| R: CCCAATACGAGGCATATGTGA | ||||
| mTb052 | F: TGACAGTGAAAAGTTGCCACA | (AAT)18 | 147–171 | KT715693 |
| R: TCATCGCAATATGTACACGATTG |
a
Annealing temperature for all loci was 55°C.