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. Author manuscript; available in PMC: 2017 Mar 1.

Published in final edited form as: Anal Biochem. 2015 Dec 29;496:79–93. doi: 10.1016/j.ab.2015.12.013

Table 1.

Dynamic range calculations for MST data.

	B* = 10 nM; signal/noise = 50
	K_D (nM)
	0.3	0.4	0.5	0.6	0.7	0.8	0.9	1.0

successful trials^a	8,301	9,566	9,898	9,975	9,998	10,000	10,000	10,000

mean value	0.3	0.4	0.5	0.6	0.7	0.8	0.9	1.0

C.I.^b	[0.2, 0.4]	[0.3, 0.5]	[0.4, 0.7]	[0.5, 0.8]	[0.5, 0.9]	[0.6, 1.0]	[0.7, 1.1]	[0.8, 1.2]

C.I. (ESP)^c	[0.1, 0.6]	[0.2, 0.7]	[0.3, 0.8]	[0.3, 1.0]	[0.4, 1.1]	[0.5, 1.2]	[0.6, 1.3]	[0.7, 1.4]

B* = 2 nM; signal/noise = 10

successful trials	N.D.	N.D.	7,206	8,155	8,742	9,051	9,368	9,621

mean value	-	-	0.6	0.7	0.8	0.9	1.0	1.1

C.I.^b	-	-	[0.2, 0.9]	[0.3, 1.1]	[0.4, 1.2]	[0.4, 1.4]	[0.5, 1.5]	[0.6, 1.6]

C.I. (ESP)^c	-	-	[0.2, 1.8]	[0.2, 2.0]	[0.3, 2.2]	[0.3, 2.4]	[0.3, 2.6]	[0.4, 2.8]

^a

out of 10,000 total trials

^b

Confidence interval calculated from the histogram of fitted values

^c

Confidence interval calculated as the respective means of ESP limits for all successful trials