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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Anal Biochem. 2015 Dec 29;496:79–93. doi: 10.1016/j.ab.2015.12.013

Table 2.

Parameters for kinetics simulations

Simulation No.
1 2 3
KD (pM) 1,000 50 1
kon (M−1s−1) 1.0 × 106 1.0 × 106 1.0 × 106
koff (s−1) 1.0 × 10−3 5.0 × 10−5 1.0 × 10−6
B*(pM) 10,000 10 10