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. Author manuscript; available in PMC: 2017 May 15.
Published in final edited form as: Cancer Res. 2016 Mar 24;76(10):2876–2881. doi: 10.1158/0008-5472.CAN-15-3432

Figure 2.

Figure 2

Differential in vitro effects of GSC co-culture and exposure to EVs.

A. Workflow depicting labelling and co-treatment of GSCs and GSC EVs (top). Representative micrographs of GSC spheroids releasing labelled EVs (bottom). Arrows indicate EVs shed by P and M GSCs. Scale bar 50μm.

B. EVM promote growth of P GSCs. Representative micrographs of GSC spheroids co-cultured with EVs (left) and quantification of spheroid frequency (right). * P < 0.05. Scale bar 100μm.

C. Workflow depicting co-culture of labelled GSCs (left). Representative micrographs of GSC spheroids and transfer of EVs between GSCs in the spheroid (right). Arrows indicates EV internalization. Scale bars 100μm (left) and 25μm (right).

D. M GSCs promote growth of P GSCs. Quantification of cell growth in mono- and co-culture. * P < 0.05.

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