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. Author manuscript; available in PMC: 2016 Oct 25.
Published in final edited form as: Nat Immunol. 2016 Apr 25;17(6):695–703. doi: 10.1038/ni.3456

Figure 4. Tcf1 has intrinsic HDAC activity.

Figure 4

(a) Deacetylation assay using IVT HDAC1, Tcf1 or Runx3. AU, arbitrary unit. (b) Deacetylation assay of IVT HDAC1 and Tcf1 in the presence of various HDAC inhibitors at indicated concentrations, with the activity of untreated HDAC1 or Tcf1 set as 1 and the relative activity of inhibitor-treated proteins normalized accordingly. (c) Deacetylation assay using purified His-tagged recombinant Tcf1 or Phf8 segment at indicated protein amounts. (d) Deacetylation assay of purified recombinant Tcf1 (3.2 µg) in the presence of HDAC inhibitors. Data are from ≥ 3 experiments (a, b and d, means ± s.d.) or average from 2 experiments (c). ns, not statistically significant; *, p<0.05; **, p<0.01; and ***, p<0.001 compared with untreated proteins, by Student’s t-test.