Table 1.
EC50 and Maximal Amplitude of Dose Response Curves.
| Genotype | Cell Type |
Daylength
LD:DD |
PDF EC50
ZT4 |
PDFEC50
ZT22 |
Max Ampl PDF
ZT4 |
Max Ampl PDF
ZT22 |
|---|---|---|---|---|---|---|
| Pdf > Epac50A | s-LNV | 12:12 | 5.4 × e-8 | 2.1 × e-7** | 17.4 ± 1.7 | 17.5 ± 1.2 |
| Pdf > Epac50A | s-LNV | 08:16 | 2.9 × e-8 | 2.6 × e-7** | 20.1 ± 1.8 | 17.1 ± 0.9 |
| Pdf > Epac50A ; Pdfr | s-LNV | 12:12 | 8.8 × e-8 | 4.1 × e-7** | 22.8 ± 1.6 | 23.2 ± 1.6 |
| pdfr[han], Pdf > Epac50A ; Pdfr | s-LNV | 12:12 | 1.0 × e-7 | 5.1 × e-7* | 23.9 ± 1.6 | 22.6 ± 1.3 |
| Pdf > Epac50A; pdf[01] | s-LNV | 12:12 | 9.9 × e-8 | 3.6 × e-7* | 17.3 ± 1.7 | 16.9 ± 1.4 |
| c929 > Epac50A | l-LNV | 12:12 | 2.0 × e-8 | 3.2 × e-8 | 18.2 ± 1.7 | 21.9 ± 1.9 |
| c929 > Epac50A | SE2 | 12:12 | 2.6 × e-8 | 2.4 × e-8 | 33.9 ± 4.3 | 35.7 ± 5.5 |
| Pdf > Epac50A ; ralaDN | s-LNV | 12:12 | 3.2 × e-7 | 4.4 × e-7 | 16.0 ± 1.1 | 16.9 ± 1.6 |
| Pdf > Epac50A ; ralaCA | s-LNv | 12:12 | 8.2 × e-8 | 9.1 × e-8 | 18.7 ± 1.8 | 16.8 ± 1.1 |
|
PDF EC50
CT4 |
PDF EC50
CT22 |
Max Ampl PDF
CT4 |
Max Ampl PDF
CT22 |
|||
| Pdf > Epac50A | s-LNV | 12:12 | 1.2 × e-7 | 3.7 × e-7** | 17.8 ± 1.2 | 19.0 ± 1.3 |
|
DA EC50
ZT4 |
DA EC50
ZT22 |
Max Ampl DA
ZT4 |
Max Ampl DA
ZT22 |
|||
| Pdf > Epac50A | s-LNV | 12:12 | 4.7 × e-6 | 2.2 × e-5** | 15.3 ± 1.2 | 14.7 ± 2.8 |
| Pdf > Epac50A | l-LNV | 12:12 | 5.1 × e-6 | 4.7 × e-6 | 15.3 ± 1.3 | 16.0 ± 1.3 |
| Pdf > Epac50A ; ralaDN | s-LNV | 12:12 | 2.9 × e-5 | 4.8 × e-5 | 13.6 ± 1.5 | 14.4 ± 1.9 |
| Pdf > Epac50A ; ralaCA | s-LNv | 12:12 | 5.7 × e-6 | 6.4 × e-6 | 14.0 ± 1.4 | 13.6 ± 2.0 |
*
Significant differences in EC50 values (p>0.001), between ZT4 vs ZT22 and between CT4 vs. CT22 were only observed in s-LNv, with the exception of those expressing ralaDN or ralaCA. No Maximal Amplitude daily differences were observed between ZT4 and ZT22 in any cells.
**
Significant differences in EC50 values (p>0.0001), between ZT4 vs ZT22 and between CT4 vs. CT22 were only observed in s-LNv, with the exception of those expressing ralaDN or ralaCA. No Maximal Amplitude daily differences were observed between ZT4 and ZT22 in any cells.