Establishment of glioblastoma CSC organoids. A, Micrograph of IN528 tumorspheres, scale bar = 400 µm. B–E, Parallel IN528 tumorsphere (B, C) or organoid cultures (D, E) for 2 and 6 weeks, scale bar = 1000 µm. F, Mosaic image of multiple low-power (4×) microscope fields showing organoid growth and smaller satellite spheres. G, IN528 organoid prior to embedding and sectioning. U.S. nickel for scale. H – J, Images of 6-well plate wells containing IN528 tumorspheres (H) or organoids (I, J) at indicated time points.