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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: Cancer Res. 2016 Feb 19;76(8):2465–2477. doi: 10.1158/0008-5472.CAN-15-2402

Figure 6.

Figure 6

Organoid rim non-CSCs are radiosensitive. A–D, Immunofluorescence imaging of cleaved Caspase 3 protein and SOX2 protein in IN528 organoids 96 hours after 3 Gy irradiation. White arrows indicate cleaved Caspase 3 positive cells. Scale bars = 200 µm (A, C) or 100 µm (B, D). E–G, Total cells and cells positive for SOX2 (E), cleaved Caspase 3 (F), and both (G) were blindly counted from 3 non-overlapping high power fields within the indicated organoid regions. Student's t-Test; *, p < 0.02; **, p < .001.