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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Mol Cancer Ther. 2016 Mar 3;15(5):1053–1062. doi: 10.1158/1535-7163.MCT-15-0828

Figure 4. PKA regulators did not affect LMB-11 activity; H89 inhibited S6K1 activity.

Figure 4

A–B. KOPN-8 cells were treated with indicated concentrations of PKI (A) or Dibutyl-cAMP (B) for 1 hour and then with indicated concentrations of LMB-11 for 72 hours. Cell viability was measured by WST8 assays. C. KOPN-8 cells were treated with 10 μM H89 or vehicle (Con) for the indicated times. Total cell lysates were analyzed by Western Blot for anti-phospho-S6-Kinase 1 (p-S6K1(T389)), anti-total S6K1, anti-p-CREB(S133)/p-ATF-1, anti-p-ribosomal protein S6 (p-rpS6(S240/244)), anti-total rpS6, p-GSK3β(S9), anti-total GSK3β, anti-MCL1 and Actin.