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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Neuropharmacology. 2016 Jan 6;105:114–123. doi: 10.1016/j.neuropharm.2015.12.026

Figure 4. MasRs are expressed on GABAergic neurons in the BLA.

Figure 4

(A) Atlas section corresponding to the level of sections used for in situ hybridization. The red highlighted area indicates the BLA. (B) 5X image of a coronal section through the BLA (dotted outline) of a GABA reporter mouse (Green, tdTomato signal representing vGAT). (C) 20X image and (C inset) 40X image of GAD1 mRNA (depicted as punctate magenta dots) frequently co-localizing with tdTomato signal representing vGAT (green), indicating that the reporter gene accurately tracks GABA synthesizing neurons. (D) 20X images and (D inset) 63X image of tdTomato signal representing vGAT (green) with DapB mRNA (negative control, undetectable). (E) 20X images and (E inset) 63X image of tdTomato signal representing vGAT (green) with Ubc mRNA (positive control, depicted as punctate red dots). (F) 20X image and (F inset) 63X image of mRNA for the MasR (depicted as punctate red dots) frequently co-localizing with tdTomato signal representing vGAT (green), demonstrating MasR mRNA is expressed in GABAergic neurons in the BLA. Signals of tdTomato were colored as green for better display of the collocalizations.