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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Cancer Res. 2016 Mar 24;76(9):2791–2801. doi: 10.1158/0008-5472.CAN-15-1975

Figure 3.

Figure 3

Hpa2 over expression is associated with decreased tumor vascularity. A. Immunostaining. Sections of tumor xenografts produced by control (#3, #5) and Hpa2 over expressing (#6, #64) cell clones were subjected to immunostaining applying anti-Hpa2 (upper panels), anti-LYVE-1 (second panels), anti-CD31 (third panels), and anti-HIF-1α (fourth panels) antibodies. Note, reduced tumor vascularity and increased tumor hypoxia following Hpa2 over expression. Original magnifications: upper, third and fourth panels ×40, second panels ×20. Quantification of lymph (LYVE-1-positive) and blood (CD31-positive) vessels is shown graphically in the lower panels. B–C. Real time PCR. Total RNA was extracted from the indicated cell clones and corresponding cDNAs were subjected to quantitative real-time PCR analyses. Expression of VEGF-C (B) and VEGF-A (C) are presented graphically in relation to the levels in control clone #3, set arbitrarily to a value of 1.