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. Author manuscript; available in PMC: 2016 Oct 18.
Published in final edited form as: Nat Immunol. 2016 Apr 18;17(6):677–686. doi: 10.1038/ni.3434

Figure 6. Silencing of miR-33 and miR-33* enhances Mtb targeting by the autophagy machinery.

Figure 6

(a) Immunofluorescence (IF) imaging of peritoneal macrophages infected with GFP-tagged H37Rv Mtb (green), p62 (red) and DAPI (blue) for 24 h and treated with control (ctrl) miR, anti-miR33 or anti-miR33*, or both. Quantification of p62 co-localization with Mtb shown at right. (b) IF imaging of peritoneal macrophages infected with GFP-tagged LC3 (green), H37Rv Mtb (red) and DAPI (blue) for 24h and treated with control (ctrl) miR, anti-miR33 or anti-miR33*, or both. Quantification of LC3 co-localization with Mtb shown at right. (c–d) IF imaging of (c) p62 (green) or (d) LC3 in wild type (WT) and Mir33−/−macrophages infected with DsRed-H37Rv or DsRed-ΔesxA Mtb strains. Quantification is shown at right. *P≤0.1, **P≤0.05 (one-way ANOVA (a–b), two-way ANOVA (c–d)). Data are from one experiment representative of 2 (a, b, d) or 3 (c) independent experiments with similar findings. Scale bar = 10 μm (a–d).

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