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. Author manuscript; available in PMC: 2017 Jun 3.
Published in final edited form as: Biochem Biophys Res Commun. 2016 Apr 27;474(3):469–475. doi: 10.1016/j.bbrc.2016.04.139

Figure 1. Transient induction of Mesp1 within a defined window promotes both hematopoietic and cardiac differentiation.

Figure 1

(A) Scheme depicting the protocol used to evaluate the hematopoietic and cardiac outcomes of Mesp1 induction by Dox from day 2.25–3.25 on ES cell differentiation.

(B–D) FACS analysis showing that a defined transient pulse of Mesp1 promoted mesoderm patterning (Flk-1+ and/or PDGFRα+) by day 4 (B), hematopoietic differentiation (c-Kit+/− CD41+) by day 6 (C), and cardiac differentiation (cTnT+) by day 8.