Figure 4. Signaling proteins involved in proliferation and programmed cell death are altered with PTP4A3 knockdown.
Two TNBC cell lines (MDA MB231 and HCC1143), and two ER-positive cell lines (MCF-7 and T47D) were transfected with PTP4A3 or control siRNA. After 72hrs cell were harvested and lysed for Western Blot analysis. A) Cleaved caspase 7 is increased after knockdown of PTP4A3 in TNBC cell lines only. B) ERK1/2 phosphorylation and protein levels are reduced after knockdown of PTP4A3. C) Phosphorylation of p38 is reduced after knockdown of PTP4A3.