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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Cancer Res. 2016 Feb 1;76(7):1892–1903. doi: 10.1158/0008-5472.CAN-15-2328

Figure 6. Mutual amplification between the Src-p38-Noxa and TNFα-RIP1/3-MLKL pathways.

Figure 6

(A, B). Cell cytotoxicity of indicated cells after 24 hours of cystine deprivation in the presence or absence of rotenone (A) or Necrox-5 (Nec-5, B) at indicated concentrations (n=4, p<0.005).

(C). Western blots analysis of p-Src, p-p38 and Noxa protein after 16 hours of cystine deprivation in the presence different inhibitors.

(D, E). Immunoblotting of pho-p38 and Noxa protein expression in 786-O shScr and shNoxa (D) or shTNFα (E) cells after 18 hours cystine deprivation.

(F, G, H). The activities of CysDep (A) or TNFα (F) or CysDep (G) gene signature and their correlation (H) in a dataset of normal tissue and ccRCC with VHL gene status (WT or Mut).