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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Cancer Res. 2016 Feb 1;76(7):1892–1903. doi: 10.1158/0008-5472.CAN-15-2328

Figure 7. Low doses of erastin share a similar mechanism as cystine deprivation to trigger programmed necrosis in ccRCC.

Figure 7

(A). Cell cytotoxicity (24 hours, n=4, p<0.001) and crystal violet staining (2 days) of RCC4 Vec and VHL cells treated with indicated concentrations of erastin.

(B). Western blots analysis of Noxa protein induction in RCC4 Vec and VHL cells treated with indicated concentrations of erastin.

(C). Cell cytotoxicity (24 hours, n=3, p<0.05) of 786-O Vec, VHL and different shRNA sub-lines cells treated with indicated concentrations of erastin.

(D). Model of the distinct signaling components triggered by cystine deprivation in VHL-deficient and VHL-restored ccRCC.