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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: Cancer Res. 2016 Feb 18;76(8):2443–2452. doi: 10.1158/0008-5472.CAN-15-2299

Figure 4. TRIM3 restricts active NICD transport to nucleus in glioma stem cells.

Figure 4

(A) Western blots using total protein extract from N08-74 (control, TRIM3 expressed and Vector Control) and 13113 (control, TRIM3 expressed and Vector Control) shows that full-length NOTCH1 and active NOTCH1 (NICD) remain unchanged in their expression levels following the expression of TRIM3. (B,C,D,E,E1) Immunocytochemistry data shows TRIM3 expression enhances the nuclear localization of NICD in N08-74 glioma cells. (F,G) Western blot of NICD from cytosolic and nuclear extract supports the immunocytochemical data, showing that TRIM3 expression reduces NICD localization within the nucleus. R = 3 replicates. Scale bar: 5 um (B,C,D,E). VC = Vector Control.