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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: Cancer Res. 2016 Feb 18;76(8):2094–2104. doi: 10.1158/0008-5472.CAN-15-2662

Fig 2. In vitro and in vivo kinetics of EMT initiation.

Fig 2

a-g. In vitro time-lapse imaging of EMT initiation in the cultured tumor cells. Sorted pure RFP+ tumor cells from the transgenic mice were cultured in medium with 10% FBS; GFP signal and bright field view of the cells were taken every 2 hours; the arrow indicates cell turned to GFP+ over time in one imaging field. h. The quantification of the GFP fluorescence intensity showing the increase of GFP signal over time (4.08±0.95-fold increase after 8-14h, p<0.001). i. The quantification of tumor cell axial ratio showing the increase of axial ratio after EMT (2.84±0.46 vs 1.19±0.13, p<0.001). j-l. Capture of EMT initiation in vivo. As the arrow indicates, one green tumor cell emerged during the in vivo tumor imaging. m. The quantification of GFP fluorescence intensity showing the increase of GFP signal over time (1.69±0.19-fold increase after 4-6h, p<0.001). n. The quantification of tumor cell axial ratio showing the elongation of the EMT cells after 4-6h (1.98±0.06 vs 1.11±0.05).