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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Cancer Res. 2016 Mar 4;76(9):2600–2611. doi: 10.1158/0008-5472.CAN-15-1758

Figure 3.

Figure 3

Both GPER1 and mERα are involved in estrogen-dependent PAK1 activation. MCF-7 and TMX2-28 cells were treated with E2 and either WCL were probed with indicated Abs (A) or in vitro kinase assay was performed (B). (C–D) MCF-7 and SKBR3 cells were treated with E2 and either WCL were probed with the indicated Abs (C) or in vitro kinase assay was performed (D). (E) MCF-7 PAK1 WT cells were transfected with control (scr), ERα, GPER1 (GPR) or ERα+GPER1 (ER/G) siRNAs. HA-PAK1 was IP’d and subjected to in vitro kinase assay. (F) TMX2-28 PAK1 WT cells were transfected cDNAs encoding GPER1 (GPR), ERα, or ERα+GPER1 (ER/G). HA-PAK1 was IP’d and subjected to the in vitro kinase assay.