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Hemi-hypothalamic coronal sections through the arcuate nucleus (Arc) of castrate adult male monkey showing immunopositive substance P neurons (insets) revealed with the Eskay antibody using Ni-DAB (left panel) and immunofluorescence (right panel; confocal projection 40×; 1 µm optical sections). Insets show at higher magnification corresponding neurons demarcated by dotted boxes. 3V, third ventricle; scale bar, 100µm.
