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Confocal projections (10×; 1 µm optical sections) of double immunofluorescence images for substance P (red) and kisspeptin (green) of a hypothalamic section from the anterior (upper panels) and posterior (lower panels) arcuate nucleus of a castrate adult male monkey. The sections shown in the right hand panels were stained after the substance P antibody was pre-adsorbed with synthetic peptide at a concentration of 5 µg/ml. 3V, third ventricle; scale bar, 100µm.
