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Confocal projection (40×; 1 µm optical sections) showing the relationship between substance P (SP, left panel, red) and kisspeptin (KP, center panel, green) neurons in a coronal hemi-section of the arcuate nucleus in a castrate adult male monkey. Merged projection indicating co-localization of both peptides in one out of the two substance P neurons present in the Arc (right panel). Insets show at higher magnification corresponding neurons demarcated by dotted boxes. Scale bar, 50µm.
