Table 1. Primary antibodies used in immunohistochemistry.
| Antibody | Source | Dilution |
|---|---|---|
| Goat anti-human HNF1α antibody | Santa Cruz Biotechnology, Santa Cruz, CA, USA | 1:100* |
| Goat anti-human HNF4α antibody | Santa Cruz Biotechnology, Santa Cruz, CA, USA | 1:100* |
| Goat anti-mouse osteopontin antibody | R&D Systems, Minneapolis, MN, USA | 1:100 |
| Mouse anti-proliferating cell nuclear antigen antibody clone PC10 | Dako Japan, Tokyo, Japan | 1:1000 |
| Rabbit anti-calf keratin antiserum | Dako, Carpinteria, CA, USA | 1:200 |
| Rabbit anti-HES1 antibody (D6P2U) | Cell Signaling Technology Japan, Tokyo, Japan | 1:100 |
| Rabbit anti-human HNF4α antibody | Santa Cruz Biotechnology, Santa Cruz, CA, USA | 1:500* |
| Rabbit anti-human phospho-histone H3 (Ser10) antiboby | Cell Signaling Technology Japan, Tokyo, Japan | 1:100 |
| Rabbit anti-human SOX9 antibody | Millipore Corporation, Temecula, CA, USA | 1:500* |
| Rabbit anti-human topoisomerase IIα antibody [EP1102Y] | abcam, Tokyo, Japan | 1:100* |
| Rabbit anti-mouse carbamoylphosphate synthase I (CPSI) antibody | Nitou et al., 2002 | 1:500 |
| Rabbit anti-mouse Ki67 antibody | Novus Biologicals, Littleton, CO, USA | 1:500* |
| Rat anti-mouse cytokeratin no.19 antibody | Developmental Studies Hybridoma Bank, Iowa City, IA, USA | 1:100* |
| Rat anti-mouse Ep-CAM antibody | Developmental Studies Hybridoma Bank, Iowa City, IA, USA | 1:200** |
*Antigen retrieval treatment (TE treatment for 10 min) was carried out in paraffin sections. **Frozen sections fixed in MEMFA were used.