Table 1.
Bacterial strains and plasmids used in this work.
| Strain or plasmid | Relevant characteristics | Reference |
|---|---|---|
| Escherichia coli strains | ||
| Top10 | Cloning strain | Invitrogen |
| S17-1 | Conjugation strain | Simon et al., 1983 |
| ET8000 | Wild-type strain | MacNeil et al., 1982 |
| Herbaspirillum seropedicae strains | ||
| SmR1 | Parental strain, Nif+, SmR | Souza et al., 2000 |
| ΔphaP1 | Chromosomal deletion of phaP1 | Tirapelle et al., 2013 |
| ΔphaP2 | Chromosomal deletion of phaP2 | This work |
| ΔphaP1.2 | Chromosomal deletion of phaP1 and phaP2 | Tirapelle et al., 2013 |
| ΔphaC1 | Chromosomal deletion of phaC1 | Tirapelle et al., 2013 |
| Plasmids | ||
| pTZ18R | Cloning plasmid | Mead et al., 1986 |
| pTZ57R/T | T/A cloning plasmid | Fermentas |
| pDK6 | Expression vector tac promoter lacIq, KmR | Kleiner et al., 1988 |
| pMMS31 | Derivative of pDK6 encoding PhbF from H. seropedicae SmR1 | Kadowaki et al., 2011 |
| pMP220 | RK2 derivative, low-copy number, promoterless lacZ containing vector used to construct transcriptional fusions; TcR | Spaink et al., 1987 |
| pEFT11 | pMP220 harboring the 5′-flanking region of phaP1 cloned upstream of lacZ | This work |
| pEFT12 | pMP220 harboring the 5′-flanking region of phaP2 cloned upstream of lacZ | This work |
| pK18mobsacB | Suicide vector; KmR, sacB, mobilizable plasmid | Schafer et al., 1994 |
| pEFT13 | Deletion product ΔphaP2 cloned into the pK18mobsacB | This work |
| pBBR1MCS3 | Broad-host-range vector | Kovach et al., 1995 |
| pLPA01 | pBBR1MCS3 harboring phaP1 of H. seropedicae. Over-expression of PhaP1 | This work |
| pLPA02 | pBBR1MCS3 harboring the phaP2 of H. seropedicae. Over-expression of PhaP2 | This work |