Fig. 5.

Oligodendrocyte-lineage cells proliferate in the damaged striatum, particularly in the peri-hematoma region. a Representative images show oligodendrocyte-lineage cells labeled for Olig2 (green), proliferating cells stained with an antibody against Ki67 (red), and nuclei labeled with DAPI (blue). Images were taken from the ipsilateral side in the peri-hematoma region (left panels), surrounding striatum (middle), and SVZ (right). Scale bar = 50 μm and applies to all panels. b Higher-magnification images of typical single-, double-, and triple-stained nuclei in each region at 3 days after ICH. c Density of proliferating oligodendrocyte-lineage cells (i.e., cells with Olig2⁺Ki67⁺ nuclei) in the peri-hematoma and surrounding striatum from 1 to 28 days after ICH. d Percentage of oligodendrocyte-lineage cells that were proliferating. Values are mean ± SEM from three rats at each time. Statistical comparisons were based on a one-way ANOVA followed by Tukey’s post-hoc test and show differences: † from the preceding time point; one symbol p < 0.05, two symbols p < 0.01, three symbols p < 0.001. Additional significant differences in the density of proliferating oligodendrocyte-lineage cells that are not indicated with symbols were as follows: (i) in the peri-hematoma, 1 vs 7 days, 3 vs 14 days, and 28 vs 3 and 7 days; and (ii) in the surrounding striatum, 3 vs 14 and 28 days. For the percentage of oligodendrocyte-lineage cells that were Ki67⁺, all time points were significantly different from 3 days in both the peri-hematoma and surrounding striatum