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. 2016 Jan 8;7:192–208. doi: 10.1007/s12975-015-0445-3

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 6 — Oligodendrocyte precursor cells (OPCs) increase preferentially in the peri-hematoma and inside white-matter bundles. a OPCs were identified as cells with Olig2⁺ nuclei (red) that were double-labeled with an antibody against NG2 chondroitin sulfate proteoglycan (green). Representative images were taken from the contralateral striatum, and the peri-hematoma region and the surrounding striatum on the ipsilateral side. The higher-magnification inset shows a typical OPC, with an Olig2⁺ nucleus surrounded by NG2. Scale bar = 50 μm (main panels), 10 μm (inset). b Time-dependent changes in density of OPCs (Olig2⁺NG2⁺ cells). Values represent mean ± SEM from three rats at each time point, and the dashed line represents the mean for 1-day saline controls (n = 3). Statistical comparisons were based on a one-way ANOVA followed by Tukey’s post-hoc test. Comparisons show differences: † from the preceding time point, # from the 1-day saline control; one symbol p < 0.05, two symbols p < 0.01, three symbols p < 0.001, four symbols p < 0.0001. Additional significant differences that are not shown with symbols are as follows: (i) in the peri-hematoma, 7 vs 1 and 28 days, 3 vs 14 and 28 days; and (ii) in the surrounding striatum, 3 vs 14 and 28 days. c Representative images from sagittal sections taken from the peri-hematoma region were triple-stained for Olig2 (red), NG2 (green), and myelin basic protein (MBP; blue). Scale bar = 50 μm and applies to all panels. d Density of OPCs (Olig2⁺NG2⁺ cells) inside or outside white-matter bundles in the peri-hematoma region. Values are mean ± SEM from four rats at each time point after ICH. The dashed lines indicate mean levels for 1-day saline controls (n = 3) for OPCs inside (red) and outside the bundles (black). Statistical comparisons were based on a two-way ANOVA, followed by Tukey’s post-hoc test. Comparisons show differences: † from the preceding time point, # from the saline control, * between those inside and outside the bundles; one symbol p < 0.05, two symbols p < 0.01, three symbols p < 0.001

Fig. 6 — Oligodendrocyte precursor cells (OPCs) increase preferentially in the peri-hematoma and inside white-matter bundles. a OPCs were identified as cells with Olig2⁺ nuclei (red) that were double-labeled with an antibody against NG2 chondroitin sulfate proteoglycan (green). Representative images were taken from the contralateral striatum, and the peri-hematoma region and the surrounding striatum on the ipsilateral side. The higher-magnification inset shows a typical OPC, with an Olig2⁺ nucleus surrounded by NG2. Scale bar = 50 μm (main panels), 10 μm (inset). b Time-dependent changes in density of OPCs (Olig2⁺NG2⁺ cells). Values represent mean ± SEM from three rats at each time point, and the dashed line represents the mean for 1-day saline controls (n = 3). Statistical comparisons were based on a one-way ANOVA followed by Tukey’s post-hoc test. Comparisons show differences: † from the preceding time point, # from the 1-day saline control; one symbol p < 0.05, two symbols p < 0.01, three symbols p < 0.001, four symbols p < 0.0001. Additional significant differences that are not shown with symbols are as follows: (i) in the peri-hematoma, 7 vs 1 and 28 days, 3 vs 14 and 28 days; and (ii) in the surrounding striatum, 3 vs 14 and 28 days. c Representative images from sagittal sections taken from the peri-hematoma region were triple-stained for Olig2 (red), NG2 (green), and myelin basic protein (MBP; blue). Scale bar = 50 μm and applies to all panels. d Density of OPCs (Olig2⁺NG2⁺ cells) inside or outside white-matter bundles in the peri-hematoma region. Values are mean ± SEM from four rats at each time point after ICH. The dashed lines indicate mean levels for 1-day saline controls (n = 3) for OPCs inside (red) and outside the bundles (black). Statistical comparisons were based on a two-way ANOVA, followed by Tukey’s post-hoc test. Comparisons show differences: † from the preceding time point, # from the saline control, * between those inside and outside the bundles; one symbol p < 0.05, two symbols p < 0.01, three symbols p < 0.001