Morphology of electrospun PLLA fibers. Brightfield microscopy of PLLA films (A) exhibit a uniform flat topography while aligned electrospun PLLA fibers are oriented either (B) parallel or (C) perpendicular to the long axis of the glass coverslip (bar = 1 mm). (D) PLLA films exhibit a uniform flat topography while electrospun PLLA fibers oriented either (E) parallel or (F) perpendicular to the long axis of the glass coverslip and applied electrical stimulus exhibit high degrees of alignment. (G) Images were captured using a Carl Zeiss Supra55 with Direct Write Attachment with a 5.00 mm working distance, 20 μm aperture diameter, and an accelerating voltage of 3kV. n=3, m=3, bar = 10 μm. The aligned fibers images were analyzed using ImageJ and the fibers exhibit similar density, diameter, and alignment regardless of fiber orientation (parallel (0°) or perpendicular (90°) to long axis of the glass coverslip).