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. Author manuscript; available in PMC: 2016 May 20.
Published in final edited form as: J Neural Eng. 2014 Jun 3;11(4):046002. doi: 10.1088/1741-2560/11/4/046002

Figure 2.

Figure 2

Electrical stimulation and topographical cues from aligned fibers both serve to promote neurite outgrowth. Immunofluorescent images of neurons on laminin-coated PLLA films and electrospun fibers with or without electrical stimulation are shown. Neurite outgrowth on the unstimulated, control PLLA films (A) was shorter than outgrowth emanating from electrically stimulated neurons (50 mV/mm; 8 hr) (B). Neurite outgrowth was greater on the aligned fibers (C, E) in comparison to planar films (A). Combining electrospun fibers with electrical stimulation further enhanced neurite outgrowth (D, F), relative to either the aligned fibers (C, E) or electrical stimulation alone (B). No anodal/cathodal bias was observed following electri cal stimulation on films or fibers. Scale bar = 100 μm, green = β-III-tubulin neurons, direction of the electric field is shown (+/−).