Figure 1.

R299Q γ2 AMPK Mice Develop Obesity

(A) R299Q allelic discrimination plot from hepatic cDNA.

(B and C) Isolated hepatocyte basal γ2-specific (B) and total (C) AMPK activity (n = 12).

(D and E) Representative immunoblot (D) and quantitation (E) of total α AMPK^Thr172 phosphorylation from isolated hepatocytes (n = 3).

(F) Male and female appearances aged 20 weeks.

(G) Growth curves on normal chow diet (n = 7).

(H) Total body fat mass at 4 and 40 weeks (n = 4–7).

(I) Hepatic H&E staining and steatosis quantification from male mice aged 40 weeks (n = 5); magnification 100×.

(J and K) Oral glucose tolerance and area (J) under the curve (AUC) for glucose (K) at 40 weeks (n = 9). (J) ^∗p < 0.05 versus WT. ^∗∗p < 0.01 Het versus WT. ζ p < 0.001 Homo versus WT.

(L and M) Insulin tolerance (L) and area above the curve (AAC) (M) for glucose at 40 weeks (n = 6). (L) ^∗p < 0.05 Het versus WT. ^∗∗p < 0.01 Homo versus WT. ζ p < 0.01 Homo versus WT.

NTC, non-template control. Data are mean ± SEM. ^∗p < 0.05. ^∗∗p < 0.01. ^∗∗∗p < 0.001. ^∗∗∗∗p < 0.0001. See also Figures S1 and S2 and Table S1.