Figure 3.

Hypothalamic Expression of γ2 AMPK and Consequences of Its Activation on ARC Neuropeptide Expression and AGRP Neuron Electrophysiology

(A) Expression pattern of Prkag2 in normal murine hypothalamus using digoxigenin ISH. Scale bar, 100 μm.

(B and C) Representative immunoblot (B) and quantitation (C) of ACC^Ser79 phosphorylation in MBH (n = 6).

(D–F) ARC gene expression of orexigenic (Agrp, D and Npy, E) and anorexigenic (Pomc, F) neuropeptides (n = 5). FPKM, fragments per kilobase per million mapped reads.

(G) Hypothalamic Agrp expression by digoxigenin ISH and quantification (n = 4). Scale bar, 100 μm.

(H–K) Current-clamp recordings from WT/NPY-hrGFP and homozygous R299Q γ2/NPY-hrGFP ARC neurons at baseline (H) and in the presence of fast synaptic inhibitors (J), together with V_m scatterplots (I and K) (n = 14). Action potential spike amplitudes truncated to demonstrate changes in V_m.

Data are mean ± SEM. ^∗p < 0.05. ^∗∗p < 0.01. ^∗∗∗p < 0.001. See also Table S2.