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. 2016 May 16;7:11550. doi: 10.1038/ncomms11550

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(a) Confocal images of mMeg-MDCK cells polarized on glass-bottom chambers, transiently transfected with Ch-DN-Rab11, allowed to internalize 647-MαHA antibody for 90 min, washed and subsequently allowed to recycle for the indicated times in the presence of 488-GαM. Each panel displays Z view (top), confocal sections at the level of the apical PM (middle-top), supranuclear region (middle-bottom) and a supranuclear confocal section displaying the signal of Ch-DN-Rab11 (bottom). Arrows denote Ch-DN-Rab11-transfected mMeg-MDCK cells. (b) Co-localization quantification and fitted curves in Ch-DN-Rab11-transfected and untransfected mMeg-MDCK cells from the same sample, for the percentage of the 647-MαHA pixels co-localizing with the 488-GαM pixels, which informs the percentage of total mMeg-HA recycled to the PM at the indicated time points. Circles represent individual cells, the continuous lines represent average and CI₉₅ and the dashed lines represent the fitted curves. (c,d) Polarized mMeg-MDCK cells were transiently transfected with GFP-DN-Rab4 and subjected to equivalent experiments to those in a and b. Scale bar, 10 μm.