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. 2016 May 17;7:11608. doi: 10.1038/ncomms11608

Figure 3. Reaction of intracellular MTSET with two cysteine residues within the DI S6.

Figure 3

(a, top) Example MTSET perfusion and voltage protocol for a closed-state modification experiment. In this particular case, a 2-ms test pulse to 60 mV was used to evoke outward current; however, in some situations we used potentials to observe inward current. (a, bottom) Exemplar traces from a closed-state modification experiment using L437C channels. The concentration of MTSET was 1.4 mM. (b, top) Example MTSET perfusion and voltage protocol for an open-state modification experiment. Depending on the cysteine construct, different test pulse potentials were used to evoke either outward or inward current. (b, bottom) Exemplar traces from an open-state modification experiment using L437C channels. (c) Closed-state modification traces for V440C. (d) Open-state modification traces for V440C. The concentration of MTSET was 180 μM and the test pulse was −40 mV for 10 s.