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. 2016 May 20;6:26166. doi: 10.1038/srep26166

Figure 1. Vascular injury induces reciprocal regulation of miR-30 family members and CaMKIIδ protein in rat carotid artery.

Figure 1

(a) Rat carotid artery was intraluminally injured and the protein levels of CaMKIIδ, Calponin, SM-MHC and β-actin (as loading reference) were measured by SDS-PAGE and immnuno bloting using specific antibodies. Histogram shows the quantification of protein levels normalized over β-actin. (b) Quantitative reverse transcriptase polymerase chain reaction (qPCR) results show mRNA levels of smooth muscle myosin heavy chain (SM-MHC), smooth muscle 22α, Klf4 and CaMKIIδ in the contra uninjured and injured carotid arteries 7 days after injury. (c) Complimentary sequences between rat CaMKIIδ 3′UTR and rat miR-30 family members. The expressions of miR-30 family members and miR-145 were compared between injured and uninjured carotid arteries and U6 expression was measured and used for normalization. Values shown are mean±S.E.M., n = 3 pairs and *p < 0.05 **p < 0.01 analyzed by paired t-test.