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. 2016 May 20;6:26166. doi: 10.1038/srep26166

Figure 4. Overexpression of CaMKIIδ partially rescues VSM proliferation inhibition by miR-30e.

Figure 4

Cultured VSM cells were eletroporated with scrambled miRNA or miR-30e mimic (0.2 pmol) and after 24 h, adeno-virus encoding CaMKIIδ2HA or GFP (2.5 MOI) was utilized to infect electroporated VSM cells for CaMKIIδ rescue. (a) Cell lysates were analyzed at 48 h and 72 h post eletroporation by western blot using specific antibodies for CaMKIIδ and GAPDH. Quantified data were normalized over GAPDH. (b) VSM Cell growth were examined by counting cell number 24 h, 48 h, and 72 h post electroporation. Values are shown as mean ± S.E.M., n = 3 and *p < 0.05, **p < 0.01 ***p < 0.001 analyzed by two-way ANOVA.