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. 2016 May 20;6:25917. doi: 10.1038/srep25917

Figure 1. Role of GO on haematopoietic specification.

Figure 1

(A) Schematic representation of experimental strategy. Haemangioblasts were cell sorted from 3.25-day-old ES derived embryoid bodies and were seeded on to either gelatin (GE) coated plastic plates or graphene oxide (GO) coated coverslips. At days 1, 2 and 3 cells were harvested and analysed for cell surface markers by FACS or clonogenic potential by CFU assays. (B) Brightfield images of haemangioblast cultures grown on either GE or GO at indicated days. (C) FACS analyses of haemangioblast cultures grown on either GE or GO at indicated days. (D) Percentages of CD41 positive cells in haemangioblast cultures grown on either GE or GO at indicated days (Mean, N = 6). (E) Absolute number of cells counted in haemangioblast cultures grown on either GE or GO at day 3 (N = 4). Scale bars represent 100 μm. Asterisks indicate significant differences (Paired t-test. ***p < 0.001, ****p < 0.0001).