Figure 3. Electrical stimulation activated the microRNA/Sox6 pathway in SOL.
(A) Western blotting analysis of Sox6 expression. Tubulin was used as the loading control. The triangle shows the target band. (B) qRT-PCR analysis of mir-499 and mir-208b. Values are expressed as the mean ± SEM, *P < 0.05 vs. the NC group, #P < 0.05 vs. the HS or HH group. NC: normal control group; HS: hypoxia-hypercapnia + sham stimulation group; HH: hypoxia-hypercapnia group; LFES: hypoxia-hypercapnia+ low frequency electrical stimulation group; HFES: hypoxia-hypercapnia+ high frequency electrical stimulation group.