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. 2016 May 20;6:26273. doi: 10.1038/srep26273

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(a) Control and BARD1β expressing SW480 cells were incubated with anti-BARD1 (red) and anti-BRCA1 (green) for 16 h following fluorescence secondary antibody staining. Nuclei were stained with DAPI. (b) Nuclear and cytoplasmic compartments were isolated by fractionation of stable FL BARD1 and BARD1β expressing SW480 cells. Immunoblotting for BRCA1 is indicated in the top panel, lamin B (control for nuclear fraction) and α-tubulin (control for cytoplasmic fraction) are in the middle and bottom panels respectively. (c) Stable control, full length BARD1 expressing and BARD1β expressing SW480 cells transfected with ubiquitin-FLAG and cyclin B-MYC, and treated with MG132. Detection of ubiquitin-FLAG or cyclin B-MYC was carried by immunoprecipitation with an antibody against MYC followed by immunoblot with an antibody against FLAG-tag.