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. 2016 May 20;6:26449. doi: 10.1038/srep26449

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Representative images from (a) control and (b–f) various incubation durations following one hour of 70% static strain are shown. (a1–f1) The inserts show magnified views of the nuclei. Sections were immuno-labeled with an anti-HSP70 antibody and the nuclei were labeled with DAPI. Scale bars: 10 μm. (g) Bar chart to show the mean ± SEM percentage of HSP70-positive cells (i.e., calculated from the number of HSP70-positive cells and the total number of cells) at different incubation durations. n = 10 images in each experimental group. The asterisks indicate statistically significant differences (p < 0.05).