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. 2016 May 20;6:26357. doi: 10.1038/srep26357

Figure 5. Effect of p50 mutant plasmid (C62S) on PL-induced cell growth inhibition and expression of Fas and DR4 in NSCLC cells as well as effect of Fas and DR4 siRNA transfection on PL induced cell growth inhibition.

Figure 5

(a) Cells were transfected with p50 mutant plasmid (C62S) for 24 h, then were treated with PL (10 μM) for another 24 h. Cell viability was determined by MTT assay. Data was expressed as the mean ± S.D. of three experiments. *p < 0.05 indicates significantly different from control cells. #p < 0.05 indicates significantly different from PL-treated cells. (b) Effect of p50 mutant plasmid (C62S) on the expression of DRs was determined by using Western blotting with antibodies against Fas, DR4 and β-actin (internal control). Each band is representative for three experiments. (c) Cells were transfected with Fas siRNA or DR4 siRNA for 24 h, and then were treated with PL (10 μM) for another 24 h. Cell viability was determined by MTT assay. Data was expressed as the mean ± S.D. of three experiments. *p < 0.05 indicates significantly different from control cells. #p < 0.05 indicates significantly different from PL-treated cells. (d) Effect of Fas siRNA or DR4 siRNA transfection on DNA binding activity of NF-κB was determined by EMSA as described in the materials and methods. For the cropped images, samples were run in the same gels under same experimental conditions and processed in parallel. Full-length gels are presented in Supplementary Fig. 7.